Course information | Practical Protein Biochemistry / Praktiese proteenbiochemie - 711

STEMLearn 2026 Modules

Lecturers, please note:
Non-yearbook modules (e.g. MT modules), have not been rolled over. Please send a request if any should be rolled over. Please review content and staff enrollments of 2026 modules.

Students, please note:
Most undergraduate modules are kept hidden to allow lecturers to update class content. Modules will be made visible closer to the start of classes. Please ensure registration is in order in the meantime.

2026-11412-711 Practical Protein Biochemistry / Praktiese proteenbiochemie - 711

Facilitator: Donita Africander
Facilitator: Arrie Arends
Facilitator: Ralie Louw
Facilitator: RENATE Louw-Du Toit
Facilitator: Karl Storbeck
Facilitator: Erick Strauss
Facilitator: Suleiman Sungay
Facilitator: Dawie Van Niekerk
Facilitator: MONICA NOSICELO Yanta

HOME

Announcements

Topic 1

Keeping a Laboratory Notebook (Guide)

Sections of a Laboratory Report (Guide)

Topic 2

Safety Features and Hazards (Simulation)

Lab Safety Points (Guide)

Dressing for the Lab (Simulation)

Clothing Safety (Guide)

Accidents and Incidents (Guide)

pH Meter (Simulation)

pH Meter (Video)

Weighing with Taring using a Top Pan Balance (Simulation)

Weighing with Taring using a Top Pan Balance (Video)

Weighing with Taring using an Analytical Balance (Simulation)

Weighing with Taring using an Analytical Balance (Video)

Centrifuge (Simulation)

Centrifuge (Video)

Preparing Solutions by Dilution (Guide)

Preparing Solutions by Dilution (Video)

Preparing Standard Solutions (Simulation)

Preparing Standard Solutions (Guide)

Preparing Standard Solutions (Video)

Topic 3

pH and Buffer Calculations (Guide)

Automated Pipette (Simulation)

Automated Pipette (Video)

Topic 4

Assignment 1 - PCR &amp; qPCR

WEEK 1, Day 4: qPCR

WEEK 1, Day 5: Restriction enzyme digest & electrophoresis

Assignment 2 - Plasmid isolation &amp; Restriction enzyme digest

WEEK 2, Day 1: Competent cells and Transformation

WEEK 2, Day 2: Calculating transformation efficiency and buffer preparation

Assignment 3 - Expression and Transformation

WEEK 2, Day 3: Protein expression

WEEK 2, Day 4: Cell lysis and Protein purification

Assignment 4 - Protein purification and desalting

WEEK 2, Day 5: Protein Desalting & Spectrophotometry

Size Exclusion Chromatography (Simulation)

Spectrometer Design (Simulation)

UV-Vis Spectrophotometer (Simulation)

UV-Vis Spectrophotometer (Video)

Assignment 5 - Spectrophotometry

WEEK 3, Day 2: SDS-PAGE & Western blot prep

WEEK 3, Day 3: SDS-PAGE & Western Blot Part 1 (Protein Transfer)

Assignment 6 - SDS-PAGE &amp; Western Blot

WEEK 3, Day 5: EcGR activity assay (Kinetic Characterisation)

WEEK 4, Day 2: EcGR activity assay (NEM inhibition)

Wrap-up: Report & lab book hand-in

Report Writing - Styles and Formatting (Guide)

Final Report: Due Monday 23 June @ 9h00

STEMLearn 2026 Modules

Assignment 1 - PCR & qPCR

Assignment 2 - Plasmid isolation & Restriction enzyme digest

Assignment 6 - SDS-PAGE & Western Blot